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Surveillance Program
 
Mosquito Trapping
In order to more accurately monitor the mosquito population, Hillsborough County Mosquito and Aquatic Weed Control maintains 75 different mosquito traps strategically placed throughout the county. These traps are of varying designs, designed to trap different types of mosquitoes from a variety of habitats.

Our most common trap is designed by the Centers for Disease Control, and is known as a “CDC Light Trap.” At the top of the trap, a small cooler (purple) is filled with dry ice, which slowly evaporates through a small hole drilled in the bottom of it. Mosquitoes home in on this carbon dioxide trail, thinking it is the breath of an animal and hoping for a blood meal. Just below it, underneath the disc-shaped protective cover, a small light functions to attract the mosquitoes once they are closer. As they approach the light, a tiny but powerful fan sucks them into the net basket below, where they remain until our inspectors pick up the collection the next day.


 
Back at the laboratory, our inspectors empty the trap collections, and observe the various specimens underneath a microscope or magnifying lens in order to identify their species. Using a standardized method of dividing and counting, they estimate the overall numbers of mosquitoes of each species per trap. This is very tedious work, but the information is used for a number of important purposes, not the least of which is to determine the need for treatment. Many of these trap sites have been maintained for decades, and this historical information is critically important in determining trends statewide.

Other Traps

Additionally, similar traps have been placed high in tree canopies, and are designated as “Canopy Traps” for the purpose of trapping the Culiseta Melanura mosquito. This species of mosquito is the primary culprit in the transmission of Eastern Equine Encephalitis (EEE). While this mosquito does not feed on humans, it transmits the virus from bird to bird, spreading it throughout the wild bird population. Other species of mosquitoes which feed on both birds and humans are then capable of precipitating a major outbreak of the disease in the human and horse population. Since this mosquito feeds on birds, the traps are placed high in the tree canopy. We do not generally find the Culiseta Melanura mosquito in great numbers here in Hillsborough County. Should their numbers begin to increase, our canopy traps will alert us to the possibility of a disease outbreak and we will take immediate steps to decrease the risk.

    Other uses for mosquito traps involve testing mosquitoes which have recently ingested a blood meal. For this we combine traps with sentinel chicken cages, as in the photo of the trap shown below. The hungry mosquitoes enter the cage from below, and once they have gorged themselves on blood, their natural instinct is to fly upwards. By covering the cage with screen and placing a bottle trap above the cage, we ensure that the mosquito has nowhere to go but into the trap. These engorged mosquitoes can then be crushed in the laboratory to obtain a blood pool, which is analyzed for presence of virus. Additionally, should one or more of the chickens test positive for Encephalitis virus, records of the mosquito species found in the bottle trap at that time will give us important information for research into which species of mosquitoes are capable of transmitting which viruses.

 

Similarly, the hanging chicken bottle trap, our latest innovation, not only tells us if there are Culiseta Melanura mosquitoes in the area, but the ones that have fed on the chicken in the blue barrel can then be tested for the presence of Eastern Equine Encephalitis, giving us results much more quickly than standard laboratory testing of chicken blood. Remember, once the chicken has been bitten by an infected mosquito, it must go through a four to fifteen day incubation period before the antibodies will show up in the bloodstream. By testing the mosquitoes as well as the chickens, we can get an “early warning” and respond appropriately.

These are just a few of our research activities being conducted by our “Special Projects” crew, whose charge it is to find new and innovative ways to accomplish our mission.

 

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